Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.6K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.6K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

4.1K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.1K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

9.4K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.4K
Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

988
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
988
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

169
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
169

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ENPP1 blockade with a humanized monoclonal antibody enhances renal repair after acute kidney injury.

Cell stem cell·2026
Same author

The liver regulates ectopic calcification in Abcc6-deficient models of pseudoxanthoma elasticum.

The Journal of clinical investigation·2026
Same author

B Cell Receptor's function in virus entry: Anti-SARS-CoV-2 B cell receptors can mediate viral entry in an ACE2-independent mechanism.

PLoS pathogens·2026
Same author

Phosphatidylserine receptors TIM-1 and AXL mediate tick-borne Powassan virus entry.

iScience·2025
Same author

Pharmacological targeting of the IL-17/neutrophil axis attenuates calcific deposits in rat models of calciphylaxis.

The Journal of clinical investigation·2025
Same author

Differential tropisms of old and new world hantaviruses influence virulence and developing host-directed antiviral candidates.

PLoS pathogens·2025

Related Experiment Video

Updated: Sep 26, 2025

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

20.1K

Hippo Signaling Pathway Activation during SARS-CoV-2 Infection Contributes to Host Antiviral Response.

Gustavo Garcia1, Yijie Wang2, Joseph Ignatius Irudayam1

  • 1Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.

Biorxiv : the Preprint Server for Biology
|April 20, 2022
PubMed
Summary

The Hippo signaling pathway plays a crucial antiviral role in COVID-19 pathogenesis. Targeting this pathway, including YAP, may offer new therapeutic strategies against SARS-CoV-2 infection.

More Related Videos

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

10.6K
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.2K

Related Experiment Videos

Last Updated: Sep 26, 2025

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

20.1K
Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

10.6K
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.2K

Area of Science:

  • Molecular Biology
  • Virology
  • Cell Biology

Background:

  • Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) causes COVID-19, leading to respiratory failure and multi-organ damage.
  • Viral variants pose a continuous threat, highlighting the need to understand SARS-CoV-2 infection mechanisms.
  • Limited knowledge exists regarding the molecular basis of COVID-19 and its associated immunopathogenesis.

Approach:

  • Investigated Hippo signaling pathway dynamics during SARS-CoV-2 infection.
  • Utilized COVID-19 lung tissues and human cell models (pluripotent stem cell-derived cardiomyocytes and lung air-liquid interface cultures).
  • Examined the effects of inhibiting upstream kinases (MST1/2, LATS1) and the downstream transactivator YAP.

Key Points:

  • SARS-CoV-2 infection activates the Hippo signaling pathway in lung tissues and cell cultures.
  • Inhibition of MST1/2 and LATS1 kinases enhanced viral replication, suggesting an antiviral role for Hippo signaling.
  • Pharmacological inhibition of YAP significantly reduced SARS-CoV-2 replication.

Conclusions:

  • The Hippo signaling pathway plays a direct antiviral role in SARS-CoV-2 infection.
  • This pathway is a potential therapeutic target for treating COVID-19.
  • Understanding Hippo pathway dynamics is critical for developing new antiviral strategies.