Related Experiment Video
Updated: Sep 26, 2025
07:55
Intratracheal Administration of Dry Powder Formulation in Mice
Published on: July 25, 2020
11.0K
Development of a Spray-Dried Formulation of Peptide-DNA Nanoparticles into a Dry Powder for Pulmonary Delivery Using
Miftakul Munir1,2, Vicky L Kett1, Nicholas J Dunne1,3,4,5,6,7,8,9
1School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK.
Pharmaceutical Research
|April 20, 2022
Summary
Spray drying optimization produced functional RALA/pDNA nanoparticles for pulmonary gene therapy. Key parameters like mannitol concentration were identified to create effective dry powders for lung treatments.
Area of Science:
- Biotechnology
- Nanotechnology
- Pulmonary Drug Delivery
Background:
- Pulmonary gene therapy offers potential for lung disease treatment.
- Spray drying is a key method for producing inhalable powders.
- This study focused on optimizing spray drying for RALA/pDNA nanoparticles.
Purpose of the Study:
- To determine optimal spray drying parameters for RALA/pDNA nanoparticles.
- To produce an inhalable dry powder formulation for gene therapy.
- To evaluate the biological functionality of the spray-dried nanoparticles.
Main Methods:
- Full factorial design with 19 experiments to optimize spray drying.
- Varied parameters: mannitol concentration, inlet temperature, spray rate, spray frequency.
- Assessed process yield, moisture, particle size, zeta potential, encapsulation, and DNA recovery.
Main Results:
- Mannitol concentration significantly impacted most responses, except encapsulation efficiency.
- Optimal spray drying parameters, particularly low mannitol concentrations (1-3% w/v), yielded functional RALA/pDNA nanoparticles.
- All responses showed strong dependency on experimental variables.
Conclusions:
- Optimal spray drying parameters were established for RALA/pDNA nanoparticles.
- The resulting dry powder exhibits excellent biological functionality for pulmonary gene therapy.
- This optimized formulation holds promise for treating lung pathologies.
