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Updated: Sep 26, 2025

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

Published on: December 18, 2015

Connecting past and present: single-cell lineage tracing.

Cheng Chen¹, Yuanxin Liao^1,2, Guangdun Peng^3,4,5

¹Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.

|April 20, 2022

Summary

Single-cell lineage tracing uses advanced barcoding strategies to map cell history, state, and fate. This review guides researchers in selecting and optimizing these powerful biological tools.

Keywords:

CRISPR barcodes integration barcodes polylox barcodes single-cell lineage tracing

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Published on: May 20, 2022

Area of Science:

Molecular Biology
Developmental Biology
Genomics

Background:

Understanding cell history, state, and fate is crucial for cell theory and modern biology.
Single-cell lineage tracing, combined with clonal analysis and single-cell RNA sequencing (scRNA-seq), offers new avenues for biological inquiry.
Various single-cell resolution lineage tracing strategies have emerged recently.

Purpose of the Study:

To review the development of single-cell lineage tracing techniques.
To outline the characteristics of major cell barcoding strategies.
To provide a guide for choosing and improving lineage tracing methods.

Main Methods:

Review of existing literature on single-cell lineage tracing.
Focus on three key barcoding strategies: integration barcodes, polylox barcodes, and CRISPR barcodes.
Discussion of applications in animal development and stem cell research.

Main Results:

Identified integration, polylox, and CRISPR barcodes as noteworthy strategies for lineage tracing.
Highlighted the amenability of these methods in experimentally tractable systems.
Acknowledged the need for careful implementation and further effort.

Conclusions:

Single-cell lineage tracing is a rapidly advancing field with significant potential.
Choosing the right barcoding strategy requires careful consideration of technical aspects and limitations.
Further development is needed to refine and broaden the application of these techniques.