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MicroRNA-218-5p affects lung adenocarcinoma progression through targeting endoplasmic reticulum oxidoreductase 1

Gang Chen1, Qihao Wang2, Kunyu Wang3

  • 1Internal Medicine-oncology, The First People's Hospital Of Jiashan, Jiaxing, China.

Bioengineered
|April 20, 2022
PubMed

Insights

MicroRNA-218-5p (miR-218-5p) suppresses lung adenocarcinoma (LUAD) progression by downregulating endoplasmic reticulum oxidoreductase 1 alpha (ERO1A). This miR-218-5p/ERO1A axis offers potential therapeutic strategies for LUAD.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Lung adenocarcinoma (LUAD) is a lethal malignancy with incompletely understood development mechanisms.
  • Identifying key regulatory molecules is crucial for developing effective LUAD therapies.

Purpose of the Study:

  • To investigate the role of microRNA-218-5p (miR-218-5p) in LUAD progression.
  • To elucidate the interaction between miR-218-5p and endoplasmic reticulum oxidoreductase 1 alpha (ERO1A) in LUAD.

Main Methods:

  • Bioinformatic analysis to identify differentially expressed RNAs in LUAD.
  • Cellular functional assays (viability, invasion, migration) to assess miR-218-5p effects.
  • Western blot and dual-luciferase reporter assays to confirm the miR-218-5p/ERO1A interaction.

Main Results:

  • miR-218-5p was downregulated, while ERO1A was upregulated in LUAD.
  • Enforced miR-218-5p expression inhibited LUAD cell viability, invasion, and migration.
  • miR-218-5p directly targets ERO1A, and ERO1A overexpression reversed miR-218-5p's suppressive effects.
  • The miR-218-5p/ERO1A axis was found to suppress angiogenesis in LUAD.

Conclusions:

  • The miR-218-5p/ERO1A axis plays a critical role in suppressing LUAD cell progression and angiogenesis.
  • This regulatory axis represents a potential therapeutic target for LUAD treatment regimens.
Keywords:
ERO1Alung adenocarcinomamalignant progressionmechanismmiR-218-5p

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