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Frequency histograms of three high-sensitivity cardiac troponin assays in a reference population

Hanwool Cho1, Hyunjung Kim2, Jehoon Lee3

  • 1Department of Laboratory Medicine, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Journal of Clinical Laboratory Analysis
|April 20, 2022
PubMed

Insights

Establishing 99th percentile upper reference limits (URLs) for high-sensitivity cardiac troponin (hs-cTn) assays is crucial. These cardiac troponin levels vary significantly by gender and age across different assays.

Area of Science:

  • Clinical Chemistry
  • Cardiology
  • Biomarkers

Background:

  • Cardiac troponin (cTn) is a key indicator of myocardial injury when levels exceed the 99th percentile upper reference limit (URL).
  • Accurate reference limits are essential for diagnosing cardiac conditions using high-sensitivity cTn (hs-cTn) assays.

Purpose of the Study:

  • To establish the 99th percentile upper reference limits (URLs) for three hs-cTn assays in a healthy Korean population.
  • To analyze the distribution of hs-cTn values and identify variations based on gender and age.

Main Methods:

  • Measured cTn values using three hs-cTn assays: Beckman Coulter Access hs-cTnI, Abbott STAT hs-cTnI, and Roche Elecsys hs-cTnT.
  • Analyzed log-transformed cTn values, examining frequency histograms and calculating 99th percentile URLs stratified by gender and age.

Main Results:

  • Frequency histograms of log-transformed cTn values showed a bell-shaped distribution for Beckman and Abbott assays.
  • The 99th percentile URLs varied significantly across the three assays and were influenced by gender (e.g., male/female differences) and age (e.g., older/younger groups).

Conclusions:

  • The study confirmed that 99th percentile URLs for hs-cTn assays differ based on gender and age in a healthy Korean population.
  • Bell-shaped distributions were observed for Beckman and Abbott assays, indicating assay-specific performance characteristics.
Abstract

Keywords:
99th percentile upper reference limitBeckman Coulter Access hs-cTnI assayhigh-sensitivity cardiac troponinhs-cTn

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