Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemometric-Assisted Determination of Benzo[a]pyrene (BaP) and Benzo[a]anthracene (BaA) in Water Samples Using Fluorescence and Commercial Membranes: A White Analytical Chemistry Approach.

Luminescence : the journal of biological and chemical luminescence·2026
Same author

Integrated computational modeling of chalcone-based inhibitors targeting carbonic anhydrase I and II: 3D-QSAR, molecular docking, and dynamics simulations.

Journal of computer-aided molecular design·2026
Same author

Determination of Polycyclic Aromatic Hydrocarbons and Organic Molecular Tracer Compounds in Dusts Samples from Schools in Puchuncaví and Quintero (Chile).

Molecules (Basel, Switzerland)·2026
Same author

Diurnal and Phenological Modulation of Canopy Temperature in Wheat Breeding Under Mediterranean Conditions.

Plants (Basel, Switzerland)·2026
Same author

Experimental and In Silico Studies to Unravel the Antioxidant and Antibacterial Properties of Lichen Metabolites from <i>Pseudocyphellaria compar</i> and <i>Pseudocyphellaria nudata</i>.

Antioxidants (Basel, Switzerland)·2026
Same author

Mapping Global Research Trends on Autism Spectrum Disorder: A Bibliometric Analysis of Pharmacology and Pharmacy Studies.

Pharmaceuticals (Basel, Switzerland)·2026

Related Experiment Video

Updated: Sep 26, 2025

An Aptamer-based Sensor for Unchelated GadoliniumIII
05:15

An Aptamer-based Sensor for Unchelated GadoliniumIII

Published on: January 9, 2017

7.4K

Sensitive fluorescent chemosensor for Hg(II) in aqueous solution using 4'-dimethylaminochalcone.

Marco Mellado1, Nicole Roldán2, Rodrigo Miranda2

  • 1Instituto de Investigación y Postgrado, Facultad de Ciencias de la Salud, Universidad Central de Chile, 8330507, Santiago, Chile. marco.mellado@ucentral.cl.

Journal of Fluorescence
|April 20, 2022
PubMed
Summary

This study introduces a novel chalcone-based fluorescent sensor for detecting mercury ions (Hg(II)) in aqueous solutions. The sensor demonstrates selective fluorescence quenching, offering a sensitive method for environmental and biological monitoring.

Keywords:
ChalconeMercury(II)Selective fluorescence quenchingchemosensor

More Related Videos

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
07:07

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Published on: March 12, 2015

9.8K
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

10.3K

Related Experiment Videos

Last Updated: Sep 26, 2025

An Aptamer-based Sensor for Unchelated GadoliniumIII
05:15

An Aptamer-based Sensor for Unchelated GadoliniumIII

Published on: January 9, 2017

7.4K
Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
07:07

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Published on: March 12, 2015

9.8K
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

10.3K

Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Materials Science

Background:

  • Mercury (Hg) poses significant neurotoxicity risks.
  • Fluorescent probes are crucial for visualizing cellular processes.
  • Chalcone derivatives offer underexplored fluorescent properties.

Purpose of the Study:

  • To synthesize and characterize a novel chalcone derivative as a fluorescent chemosensor.
  • To evaluate the chemosensor's selectivity and sensitivity towards mercury ions (Hg(II)).
  • To elucidate the interaction mechanism between the chalcone and Hg(II) using computational methods.

Main Methods:

  • Synthesis of chalcone (2E)-3-(4-(dimethylamino)phenyl)-1-phenylprop-2-en-1-one.
  • Photophysical property assessment.
  • Fluorescence quenching studies in aqueous media with Hg(II).
  • Determination of limit of detection (LOD) and limit of quantification (LOQ).
  • Application of Benesi-Hildebrand and Stern-Volmer equations.
  • Computational modeling of the chalcone-Hg(II) interaction.

Main Results:

  • The synthesized chalcone exhibits selective fluorescence quenching in the presence of Hg(II).
  • Key performance metrics include LOD = 136 nM and LOQ = 454 nM with a 1:1 stoichiometry.
  • Association constant (Ka) and fluorescence quenching constant (KSV) were determined.
  • Computational analysis suggests a photoinduced electron transfer (PET) blocking mechanism.

Conclusions:

  • The developed chalcone-based molecule serves as an effective fluorescent chemosensor for Hg(II).
  • The sensor's high sensitivity and selectivity are suitable for detecting mercury contamination.
  • Understanding the quenching mechanism aids in designing advanced fluorescent probes.