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Related Experiment Videos

Dissection technique for cochleas prepared for scanning electron microscopy.

S Rydmarker, D E Dunn, P Nilsson

    Scanning Electron Microscopy
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

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    The anchor technique prevents basilar membrane curling during scanning electron microscopy (SEM) sample preparation. This method improves visualization of hair cells and stereocilia in the organ of Corti for better morphological evaluation.

    Area of Science:

    • Otoacoustic Emissions
    • Auditory Neuroscience
    • Scanning Electron Microscopy

    Background:

    • Scanning electron microscopy (SEM) is crucial for studying the organ of Corti's surface morphology.
    • Basilar membrane curling during critical point drying obstructs SEM visualization of hair cells and stereocilia.

    Purpose of the Study:

    • To introduce and validate the "anchor technique" for SEM sample preparation.
    • To overcome basilar membrane curling in cochlear specimens.

    Main Methods:

    • A novel dissection method, the "anchor technique," was employed.
    • Structures above the reticular lamina were removed, preserving basilar membrane attachment to the spiral ligament and anchored bone.
    • Individual cochlear turns were mounted on a single stub for SEM evaluation.

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    Main Results:

    • The anchor technique successfully prevented basilar membrane curling during critical point drying.
    • This method improved the view of the reticular lamina and ciliary ends of hair cells.
    • Mounting individual turns on one stub facilitated SEM evaluation and photomicroscopy.

    Conclusions:

    • The anchor technique is effective in preventing basilar membrane curling for SEM analysis.
    • This method enhances the evaluation of hair cell and stereocilia morphology in the organ of Corti.
    • The technique is applicable to most mammalian cochleas, including the guinea pig.