Suppression of MIR31HG affects the functional properties of thyroid cancer cells depending on the miR-761/MAPK1 axis

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Updated: Sep 26, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.5K

Shuwang Peng1, Luyang Chen2, Zhengtai Yuan3

  • 1Department of Gastrointestinal and Thyroid and Vascular Surgery, The First Hospital of Hunan University of Chinese Medicine, Ward 22, 13th floor, Zhihe Building, No.95 Shaoshan Middle Road, Yuhua District, Changsha, 410000, Hunan, Province, China. pengshuwang@126.com.

BMC Endocrine Disorders
|April 21, 2022
PubMed
Abstract

Insights

Long non-coding RNA MIR31HG promotes thyroid cancer by targeting miR-761 and upregulating MAPK1. Inhibiting MIR31HG offers a potential therapeutic strategy for thyroid cancer treatment.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Thyroid cancer is a common endocrine malignancy.
  • Long non-coding RNA (lncRNA) MIR31HG is dysregulated in thyroid cancer.
  • The exact role of MIR31HG in thyroid cancer pathogenesis is not fully understood.

Purpose of the Study:

  • To elucidate the functional role of MIR31HG in thyroid cancer.
  • To investigate the molecular mechanisms underlying MIR31HG's action.
  • To assess MIR31HG as a potential therapeutic target for thyroid cancer.

Main Methods:

  • Quantitative real-time PCR and immunoblotting to measure MIR31HG, miR-761, and MAPK1 levels.
  • In vitro assays (MTS, EdU, flow cytometry, transwell, wound-healing) to assess cell behavior.
  • Dual-luciferase reporter assays to confirm direct interactions.

Main Results:

  • MIR31HG overexpression in thyroid cancer correlates with poor prognosis.
  • MIR31HG suppression inhibits proliferation, invasion, migration, and promotes apoptosis.
  • MIR31HG targets miR-761, which in turn targets MAPK1, creating a regulatory axis.

Conclusions:

  • MIR31HG promotes thyroid cancer progression by upregulating MAPK1 via sponging miR-761.
  • MIR31HG represents a promising novel therapeutic target for thyroid cancer.
Keywords:
Functional propertiesMAPK1MIR31HGThyroid cancermiR-761

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