Related Experiment Videos
Phage typing of mycobacteria using paper discs
The American Review of Respiratory Disease
|July 1, 1978
Summary
Phage typing of mycobacteria using paper discs requires a 100x dilution and 20 microliters of phage. Soaked discs offer larger lysis zones and remain stable for 8 weeks at 4°C.
Area of Science:
- Microbiology
- Bacteriology
- Virology
Background:
- Phage typing is crucial for identifying bacterial strains.
- Mycobacteria identification requires reliable methods.
- Standardizing phage typing protocols is essential for accuracy.
Purpose of the Study:
- To evaluate phage-impregnated paper discs for mycobacteria phage typing.
- To determine optimal conditions for phage lysis using paper discs.
- To assess the stability of phage-impregnated paper discs.
Main Methods:
- Utilized five mycobacteriophages (BG1, BK1, G37, CRI-3, LG) against Mycobacterium smegmatis 607B.
- Tested various phage dilutions, volumes (≥20 microliters), and incubation methods.
- Assessed lysis areas and stability of soaked and dried phage-impregnated paper discs.
Main Results:
- A 100x routine test dilution and at least 20 microliters of phage were necessary for lysis.
- Soaked and dried discs yielded larger lysis zones compared to 20-microliter volumes.
- Phage-impregnated discs remained stable after 8 weeks of storage at 4°C.
Conclusions:
- Phage-impregnated paper discs are suitable for mycobacteria phage typing.
- Optimized conditions include 100x dilution and ≥20 microliters of phage.
- Soaked discs demonstrate stability and efficacy for prolonged use.