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Nanoscale Diamond-Based Formulation as an Immunomodulator and Potential Therapeutic for Lymphoma
Ankush Paladhi1, Abhinandan Rej1, Debanjan Sarkar2
1Cellular Immunology Laboratory, Department of Zoology, The University of Burdwan, Burdwan, India.
Frontiers in Pharmacology
|April 21, 2022
Summary
This study shows that Heerak Bhasma (Ayu_ND), an Ayurvedic formulation, effectively treats Dalton's lymphoma in mice by stimulating the immune system. It reduces tumor growth, prevents metastasis, and improves survival by modulating immune cells and cytokines.
Area of Science:
- Integrative Medicine
- Nanomedicine
- Cancer Immunology
Background:
- Ayurvedic formulations lack comprehensive scientific validation.
- Traditional Indian medicine, including Ayurveda, holds potential for novel therapeutic agents.
- Understanding the mechanisms of Ayurvedic products is crucial for their clinical application.
Purpose of the Study:
- To investigate the anti-cancer effects of a nanodiamond-based Ayurvedic formulation, Heerak Bhasma (Ayu_ND).
- To evaluate the immunomodulatory mechanisms underlying Ayu_ND's efficacy against solid tumors.
- To assess the impact of Ayu_ND on tumor proliferation, apoptosis, metastasis, and animal survival.
Main Methods:
- Utilized a mouse model of Dalton's lymphoma (CD1 mice).
- Administered functional nanodiamond-based Ayurvedic herbomineral formulation (Ayu_ND).
- Assessed tumor volume, metastasis, survival rates, immune cell populations (dendritic cells, T cells), and cytokine profiles (TNF-α, IFN-γ, TGF-β).
Main Results:
- Ayu_ND significantly reduced tumor cell proliferation and induced apoptosis.
- Treatment led to reduced tumor volume and inhibited metastasis.
- Ayu_ND enhanced immunostimulation, promoting dendritic cell activity and TNF-α production.
- Elevated serum levels of IFN-γ and TNF-α, with downregulated TGF-β, were observed.
- Therapeutic success correlated with regulatory T cell depletion, enhanced CD8+ T-cell immunity, and prevention of T cell exhaustion.
Conclusions:
- Nanodiamond-based Heerak Bhasma (Ayu_ND) demonstrates significant anti-cancer properties against Dalton's lymphoma in mice.
- Ayu_ND exerts its therapeutic effects through potent immunomodulation, enhancing anti-tumor immune responses.
- These findings support further research into Ayurvedic formulations for cancer therapy and drug efficacy.
