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LncRNA SBF2-AS1 Facilitates Nonsmall Cell Lung Cancer Progression by Targeting miR-520a-3p
Yi Wang1, Yanzhi Zou2, Qingmei Zhang3
1Department of Respiration, The People's Hospital of Zhangqiu Area, Jinan 250200, China.
Background:
Long noncoding RNA (lncRNA) SET-binding factor 2 (SBF2) antisense RNA1 (SBF2-AS1), which acts as an oncogene in various cancers, can promote tumors progression. The study aimed to explore the role and molecular mechanism of SBF2-AS1 in nonsmall cell lung cancer (NSCLC).
Methods:
qRT-PCR was introduced to detect SBF2-AS1 and miR-520a-3p expression in NSCLC. The effects of SBF2-AS1 and miR-520a-3p on the proliferation, migration, and invasion of NSCLC cells were assessed through cell counting kit-8 (CCK-8) and transwell assay. Furthermore, the relationship of SBF2-AS1 and miR-520a-3p was verified by the RNA immunoprecipitation (RIP) assay, dual-luciferase assay, and Spearman correlation analysis.
Results:
In NSCLC tissues, SBF2-AS1 was highly expressed, while miR-520a-3p expression has decreased. The overall survival of NSCLC patients with high SBF2-AS1 expression was lower. SBF2-AS1 silencing repressed the proliferation, migration, and invasion of NSCLC cells. SBF2-AS1 directly interacted with miR-520a-3p, and a negative relationship was observed between their expression levels in NSCLC tissues. More importantly, the suppression of SBF2-AS1 silencing on the proliferation, migration, and invasion in NSCLC cells was counteracted by miR-520a-3p inhibition.
Conclusion:
SBF2-AS1 accelerated the proliferation, migration, and invasion of NSCLC cells via mediating miR-520a-3p, thus promoting NSCLC progression.
Insights
Long noncoding RNA SBF2-AS1 promotes nonsmall cell lung cancer (NSCLC) progression by enhancing cell proliferation, migration, and invasion. It directly interacts with and downregulates miR-520a-3p, contributing to NSCLC development.
Area of Science:
- Molecular oncology
- RNA biology
Background:
- Long noncoding RNA (lncRNA) SBF2-AS1 is implicated as an oncogene in various cancers.
- Its role in nonsmall cell lung cancer (NSCLC) progression requires further elucidation.
Purpose of the Study:
- To investigate the role of SBF2-AS1 in NSCLC.
- To explore the underlying molecular mechanism involving miR-520a-3p.
Main Methods:
- Quantitative reverse transcription PCR (qRT-PCR) for expression analysis.
- Cell proliferation, migration, and invasion assays (CCK-8, Transwell).
- RNA immunoprecipitation (RIP), dual-luciferase, and Spearman correlation analyses to determine molecular interactions.
Main Results:
- SBF2-AS1 was significantly upregulated in NSCLC tissues, correlating with decreased patient survival.
- SBF2-AS1 silencing inhibited NSCLC cell proliferation, migration, and invasion.
- SBF2-AS1 directly targets miR-520a-3p, with an inverse correlation in expression.
- Inhibition of miR-520a-3p reversed the suppressive effects of SBF2-AS1 silencing.
Conclusions:
- SBF2-AS1 acts as an oncogene in NSCLC by promoting proliferation, migration, and invasion.
- The mechanism involves the downregulation of miR-520a-3p.
- SBF2-AS1 facilitates NSCLC progression through the SBF2-AS1/miR-520a-3p axis.
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