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Updated: Sep 26, 2025
Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
Published on: March 24, 2015
Single Molecule Level and Label-Free Determination of Multibiomarkers with an Organic Field-Effect Transistor
Chenfang Sun1, Guangyuan Feng1, Yaru Song1
1Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, China.
Abstract:
The single molecule level determination with a transistor (SiMoT) platform has attracted considerable attention in the recognition of various ultralow abundance biomolecules, while complicated labeling and testing processes limit its further applications. Recently, organic field-effect transistor (OFET)-based biosensors are good candidates for constructing an advanced label-free SiMoT platform due to their facile fabrication process, rapid response time, and low sample volume with a wide range of detection. However, the sensitivity of most OFET-based biosensors is in the order of nM and pM, which cannot meet the detection requirements of ultralow abundance protein. Herein, a label-free SiMoT platform is demonstrated by integrating pillar[n]arene as a signal amplifier, and the detection limit can reach 4.75 aM. Besides, by simultaneous determination of α-fetoprotein, carcinoembryonic antigen, and prostate antigen, the proposed multiplexed OFET-based SiMoT platform provides a key step in reliable early cancer diagnosis.
