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Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

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Bioengineered Hierarchical Bonelike Compartmentalized Microconstructs Using Nanogrooved Microdiscs.

Isabel M Bjørge¹, Bárbara M de Sousa², Sónia G Patrício¹

¹Department of Chemistry, CICECO─Aveiro Institute of Materials, University of Aveiro, Aveiro 3810-168, Portugal.

ACS Applied Materials & Interfaces

|April 21, 2022

Summary

This summary is machine-generated.

This study presents a novel modular tissue engineering strategy for creating vascularized bonelike tissues. The approach uses microcarriers and sequential cell seeding to promote osteogenesis and prevascularization in large-scale constructs.

Keywords:

biophysical cues bottom-up tissue engineering cell self-assembly compartmentalization liquefied-core capsules nanogrooved microdiscs osteogenesis prevascularization

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Area of Science:

Regenerative Medicine
Tissue Engineering
Biomaterials Science

Background:

Vascularized large-scale tissue constructs are crucial for regenerative medicine but face challenges in nutrient diffusion and cell organization.
Current strategies often rely on cell self-organization or precise control, limiting scalability and efficacy.

Purpose of the Study:

To develop a modular and hierarchical tissue-engineering strategy for fabricating bonelike tissues with enhanced vascularization.
To address nutrient diffusion limitations and promote osteogenesis and prevascularization in large-scale constructs.

Main Methods:

Utilized disc-shaped microcarriers with nanogrooved topographical cues to guide cell behavior via mechanotransduction.
Implemented a sequential seeding strategy of adipose-derived stromal cells and endothelial cells within compartmentalized, liquefied-core macrocapsules.
Developed a self-organizing and dynamic 3D system for construct fabrication.

Main Results:

The system autonomously promoted osteogenesis and construct mineralization.
A favorable environment for prevascular-like endothelial organization was established.
Demonstrated a modular and self-organizing approach suitable for larger construct fabrication.

Conclusions:

The proposed modular and hierarchical strategy effectively produces vascularized bonelike tissues.
This approach overcomes nutrient diffusion limitations and promotes key regenerative processes.
The strategy holds potential for local regeneration applications and drug-screening platforms.