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Fangchinoline induces gallbladder cancer cell apoptosis by suppressing PI3K/Akt/XIAP axis

Jiandong Li1,2,3, Wenda Cen2,3,4, Chenhao Tong1,2,3

  • 1Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Plos One
|April 21, 2022
PubMed

Insights

Fangchinoline, a natural compound, effectively inhibits gallbladder cancer (GBC) cell growth and promotes apoptosis. This study suggests fangchinoline as a promising new drug candidate for treating GBC.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Gallbladder cancer (GBC) is a prevalent biliary tract malignancy with poor patient outcomes.
  • Novel therapeutic strategies are crucial for improving GBC prognosis.
  • Bisbenzylisoquinoline alkaloids represent a class of compounds with potential anti-cancer properties.

Purpose of the Study:

  • To investigate the anti-cancer effects of fangchinoline on gallbladder cancer cells.
  • To elucidate the molecular mechanisms underlying fangchinoline's action.
  • To evaluate the therapeutic potential of fangchinoline in preclinical models.

Main Methods:

  • In vitro studies using GBC cell lines to assess proliferation and apoptosis.
  • Techniques included Hoechst staining, TUNEL assays, and flow cytometry.
  • In vivo xenograft models were used to evaluate tumor growth inhibition.

Main Results:

  • Fangchinoline demonstrated dose-dependent inhibition of GBC cell proliferation and clone formation.
  • Fangchinoline significantly induced apoptosis in GBC cells.
  • The PI3K/Akt/XIAP anti-apoptotic pathway was suppressed by fangchinoline treatment.
  • Fangchinoline treatment inhibited xenograft tumor growth in vivo.

Conclusions:

  • Fangchinoline exhibits significant anti-cancer activity against gallbladder cancer.
  • Fangchinoline induces apoptosis through the inhibition of the PI3K/Akt/XIAP pathway.
  • Fangchinoline shows potential as a novel therapeutic agent for GBC treatment.

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