Related Experiment Video
Updated: Sep 26, 2025
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
QuEChERS extraction for quantitation of bitter acids and xanthohumol in hops by HPLC-UV
Samuel Pedro Dantas Marques1, Robert Wyn Owen2, Ana Maria Amaral da Silva3
1Instituto Federal de Educação, Ciência e Tecnologia do Ceará, Departamento de Química, Av. José de Freitas Queiroz, 5000, Quixadá, CE CEP: 63902-580, Brazil; Programa de Pós-graduação em Química, Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Campus do Pici - Bloco 935 superior - Laboratório de Produtos Naturais e Biotecnologia (LPNBio), CP: 60451-970 Fortaleza, CE, Brazil.
Abstract:
We hypothesised that QuEChERS could be successfully applied to the extraction of bitter acids and xanthohumol from hops, which would be less time consuming, cheaper, and more eco-friendly by the severe reduction of solvent use. High performance liquid chromatography was used to separate the compounds after extraction and quantitation was evaluated against standard calibration curves for bitter acids prepared from an International calibration extract (ICE-4) and an authentic standard of xanthohumol. The standard QuEChERS method was compared to mini and micro-versions including clean-up and spiking procedures. The quantitative analyzes indicate the applicability of the QuEChERS method for the quantitation of bitter acids compared to Soxhlet extraction. The statistical data confirm reproducibility of the total alpha- and beta- acids measured by the standard method and the modified mini- and micro-QuEChERS procedures. Our hypothesis is supported by the data described and is consistent with other previous methods described in the literature.
