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Updated: Sep 26, 2025

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Published on: November 26, 2018

Decellularization of Full Heart-Optimizing the Classical Sodium-Dodecyl-Sulfate-Based Decellularization Protocol.

Reem Al-Hejailan¹, Tobias Weigel², Sebastian Schürlein²

¹Department of Cell Biology, King Faisal Specialist Hospital and Research Centre, Riyadh 11211, Saudi Arabia.

Bioengineering (Basel, Switzerland)

|April 21, 2022

Summary

Optimized decellularization using sodium dodecyl sulfate created vascularized cardiac patches. These patches, containing endothelial cells and cardiac cells, showed sustained function for over 16 weeks, paving the way for preclinical studies.

Keywords:

cardiac patch decellularization dynamic culture tissue engineering vascularized scaffold

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Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

Related Experiment Videos

Last Updated: Sep 26, 2025

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Published on: November 26, 2018

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

Area of Science:

Biomaterials Science
Regenerative Medicine
Cardiovascular Engineering

Background:

Vascularized scaffolds offer advantages over traditional cell therapy for heart infarction treatment.
Decellularized native tissues provide an in vivo-like microenvironment crucial for cell survival and function.
Scaffold quality is highly dependent on the efficiency and gentleness of the decellularization process.

Purpose of the Study:

To optimize decellularization protocols for porcine hearts to create functional vascularized cardiac patches.
To compare the efficacy of sodium dodecyl sulfate (SDS) and sodium deoxycholate (SDC) in decellularization.
To evaluate the biological functionality and long-term stability of the generated cardiac patches.

Main Methods:

Two decellularization protocols using SDS and SDC were optimized for porcine hearts.
Scaffold quality was assessed via DNA content, GAG quantification, and immunohistochemistry.
Human endothelial cells, cardiomyocytes, fibroblasts, and mesenchymal stem cells were seeded onto the scaffolds.

Main Results:

SDS-based decellularization yielded scaffolds with low DNA, preserved extracellular matrix (ECM) composition, and structure.
Endothelial cell seeding resulted in functional vasculature, confirmed by a coagulation assay.
The resulting vascularized cardiac patches exhibited spontaneous contraction for over 16 weeks.

Conclusions:

Sodium dodecyl sulfate-based decellularization is superior for generating high-quality cardiac scaffolds.
The developed vascularized cardiac patches demonstrate promising potential for myocardial regeneration.
This optimized protocol represents a significant step towards the preclinical evaluation of cardiac tissue engineering strategies.