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Related Experiment Video

Updated: Sep 26, 2025

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

Portable Waveguide-Based Optical Biosensor.

Philip A Kocheril¹, Kiersten D Lenz¹, David D L Mascareñas²

¹Physical Chemistry and Applied Spectroscopy Group, Chemistry Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.

|April 21, 2022

Summary

This summary is machine-generated.

Portable engineered analytic sensor with automated sampling (PEGASUS) enables rapid, on-site diagnostics. This optical biosensor successfully detected a fluorescent conjugate at 1 nM, showcasing its fieldable potential.

Keywords:

biosensor evanescent field fluorescence portable waveguide

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Last Updated: Sep 26, 2025

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Area of Science:

Biomedical Engineering
Sensor Technology
Optical Biosensing

Background:

Rapid, on-site diagnostics are crucial for warfighter support, environmental monitoring, and global health.
Portable optical biosensors offer speed and accuracy for fieldable applications.

Purpose of the Study:

To develop a fieldable and generalizable biosensing platform.
To detail the development of the PEGASUS sensing hardware.

Main Methods:

Development of the portable engineered analytic sensor with automated sampling (PEGASUS) hardware.
Utilizing a test-bed system with identical sensing hardware and software.
Demonstrating detection via biotin-streptavidin chemistry.

Main Results:

Successful detection of a fluorescent conjugate at 1 nM concentration.
Validation of the PEGASUS platform's sensing capabilities.
Demonstration of a generalizable biosensing approach.

Conclusions:

The PEGASUS platform demonstrates potential for rapid, on-site diagnostics.
The developed hardware and software are suitable for fieldable biosensing applications.
Biotin-streptavidin chemistry can be effectively utilized for sensitive detection with PEGASUS.