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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

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Fast and Efficient Mouse Pluripotency Reprogramming Using a Chemically-Defined Medium.

Junju Huang^1,2, Xuejie Yang^2,3, Jie Wang^2,3

¹Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University, Guangzhou 511436, China.

Methods and Protocols

|April 21, 2022

Summary

This summary is machine-generated.

Researchers developed a new method to efficiently generate induced pluripotent stem cells (iPSCs) from mouse embryonic fibroblasts (MEFs). This practical protocol speeds up iPSC generation for regenerative medicine and disease modeling applications.

Keywords:

fast kinetics induced pluripotent stem cells (iPSCs)mouse embryonic fibroblasts (MEFs)reprogramming transcription factors

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The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

Area of Science:

Stem cell biology
Cellular reprogramming
Regenerative medicine

Background:

Induced pluripotent stem cells (iPSCs) offer significant potential for regenerative medicine and disease modeling.
Current methods for generating iPSCs suffer from low efficiency and slow kinetics, limiting their clinical and research applications.

Purpose of the Study:

To develop a practical and efficient workflow for the rapid induction of iPSCs from mouse embryonic fibroblasts (MEFs).
To provide a protocol easily implementable in standard cell culture laboratories for iPSC generation.

Main Methods:

Utilized a chemically defined medium, iCD1 (iPS chemically-defined medium 1), for reprogramming mouse embryonic fibroblasts (MEFs).
Established a workflow designed for rapid and efficient induction of pluripotency.

Main Results:

Successfully generated induced pluripotent stem cells (iPSCs) from mouse embryonic fibroblasts (MEFs) with improved efficiency and kinetics.
The developed protocol is practical and can be readily adopted in standard cell culture settings.

Conclusions:

The iCD1 medium and associated workflow offer a significant advancement in iPSC generation, overcoming previous limitations.
This protocol facilitates broader application of iPSC technology in cell fate research and regenerative medicine.