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Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
Published on: June 25, 2020
eDNA-based detection of the invasive crayfish Pacifastacus leniusculus in streams with a LAMP assay using dependent
David Porco1, Sylvie Hermant2, Chanistya Ayu Purnomo2
1Life Science Department-Invertebrate Zoology, Population Biology and Evolution / Fondation Faune Flore, Musée National d'Histoire Naturelle, 24, Rue Münster, 2160, Luxembourg, Luxembourg. david.porco.gm@gmail.com.
Abstract:
LAMP assays are becoming increasingly popular in the field of invasive species detection but are still underused in eDNA-based monitoring. Here, we propose a LAMP assay designed to detect the North American crayfish species Pacifastacus leniusculus in water samples from streams. The presence of P. leniusculus was detected through this new LAMP assay in all but one of the nine sites sampled. No correlation was found between ddPCR absolute concentration measurements and the number of LAMP-positive technical replicates. However, we showed that using dependent technical replicates could significantly enhance the detection sensitivity of the LAMP assay. Applied to other assays, it could improve sensitivity and thus allow for a more efficient use of eDNA-based LAMP assays for invasive species detection in aquatic ecosystems.
