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Updated: Sep 26, 2025

A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis

A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis

Published on: August 26, 2021

Advances in multiplex molecular detection technologies for harmful algae.

Hanyu Fu¹, Chunyun Zhang¹, Yuanyuan Wang¹

¹College of Oceanology, Harbin Institute of Technology (Weihai), Weihai, 264209, People's Republic of China.

Environmental Science and Pollution Research International

|April 22, 2022

Summary

This summary is machine-generated.

Harmful algal blooms (HABs) are a growing threat. New molecular methods allow for the simultaneous detection of multiple harmful algae species, improving early warning systems for environmental and human health protection.

Keywords:

Antibody chip Gene chip Harmful algae Massively parallel sequencing Multiple isothermal amplification Multiple quantitative PCR Multiplex PCR

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Area of Science:

Environmental Science
Molecular Biology
Ecotoxicology

Background:

Eutrophication is increasing harmful algal blooms (HABs) globally.
HABs pose significant risks to human health, food safety, and ecosystems.
Traditional identification methods for harmful algae are slow and unreliable.

Purpose of the Study:

To review and compare molecular detection technologies for multiple harmful algal species.
To provide insights into the principles, pros, cons, and applications of these advanced methods.
To guide the development of effective early warning systems for HABs.

Main Methods:

Review of current molecular techniques including gene chips, multiplex PCR/qPCR, next-generation sequencing, antibody chips, and isothermal amplification.
Analysis of the principles, advantages, and disadvantages of each method.
Assessment of the application progress and future prospects of multi-species detection.

Main Results:

Single-species detection methods are insufficient for complex aquatic environments.
Multiple detection technologies offer rapid, accurate, and simultaneous identification of various harmful algae.
These advanced methods are crucial for comprehensive HAB monitoring.

Conclusions:

Simultaneous detection of multiple harmful algal species is essential for effective HAB management.
Molecular techniques like gene chips and multiplex PCR/qPCR show great promise.
These technologies are vital for protecting fisheries, economic activities, the environment, and public health.