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Updated: Sep 26, 2025

Rapid Generation of Amyloid from Native Proteins In vitro
Published on: December 5, 2013
Interconversion between Serum Amyloid A Native and Fibril Conformations
Fatih Yasar1, Miranda S Sheridan1, Ulrich H E Hansmann1
1Department of Chemistry & Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, United States.
Abstract:
Overexpression of serum amyloid A (SAA) can lead to a form of amyloidosis where the fibrils are made of SAA fragments, most often SAA1-76. Using Replica Exchange with Tunneling, we study the conversion of a SAA1-76 chain between the folded conformation and a fibril conformation. We find that the basins in the free energy landscape corresponding to the two motifs are separated by barriers of only about 2-3 k T. Crucial for the assembly into the fibril structure is the salt bridge 26E-34K that provides a scaffold for forming the fibril conformation.
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