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The Role of RNA-Binding Protein HuR in Lung Cancer by RNA Sequencing Analysis
Xiong Ye1, Qiang Fu2, Hui Xiao2
1School of Clinical Medicine, Shanghai University of Medicine & Health Sciences, Shanghai, China.
Frontiers in Genetics
|April 22, 2022
Summary
Human antigen R (HuR) is overexpressed in lung cancer, promoting tumor progression. Reducing HuR levels inhibits cancer cell proliferation, migration, and invasion, highlighting its role in lung cancer development.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Human antigen R (HuR) overexpression is linked to poor survival in lung cancer patients.
- HuR stabilizes tumor-promoting gene mRNAs by binding 3'-UTR AU-rich elements.
- The specific role of HuR in lung cancer proliferation remains unclear.
Purpose of the Study:
- To investigate the role of HuR in lung cancer progression.
- To assess HuR expression levels in lung cancer patients.
- To elucidate the molecular mechanisms by which HuR influences lung cancer cell behavior.
Main Methods:
- Immunohistochemistry to assess HuR expression in clinical samples.
- Single-cell RNA sequencing to analyze gene, protein, mRNA, and lncRNA changes in HuR knockdown cells.
- Cell Counting Kit-8 and Transwell assays to evaluate proliferation, migration, and invasion.
- Propidium iodide staining for cell cycle analysis.
- Western blotting and RT-qPCR to detect PLK1 levels and half-life.
Main Results:
- HuR expression is significantly higher in lung cancer patients compared to benign lung disease patients.
- HuR knockdown (KD) in A549 cells suppressed proliferation, migration, and invasion.
- RNA sequencing revealed HuR's involvement in ribonucleoprotein complex biogenesis, spliceosome, RNA transport, and cell cycle pathways.
Conclusions:
- HuR plays a significant role in promoting lung cancer progression.
- Targeting HuR may represent a therapeutic strategy for lung cancer.
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