Facile method to synthesize fluorescent chitosan hydrogels for selective detection and adsorption of Hg²⁺/Hg

Fluorescent chitosan-based hydrogel for the selective detection and adsorption of Hg²⁺/Hg⁺ in aqueous environment was prepared through three-step synthesis strategy. NO₂-Boron-dipyrrolemethene (BODIPY) was prepared firstly, and then the -NO₂ group was reduced to -NH₂ group. Finally, the NH₂-BODIPY was introduced to chitosan by Schiff base formation reaction through bi-aldehyde. Eventually, fluorescent chitosan hydrogel was obtained. The as-prepared fluorescent hydrogel probe could detect Hg²⁺/Hg⁺ through PET mechanism with the detection limit of 0.3 μM. The recognition site which combines Hg²⁺/Hg⁺ is CN, it is just formed in the reaction with chitosan and the amino group on BODIPY. Adsorption capacity of the fluorescent hydrogel is 121 mg·g^-1, which is almost seven times of the original chitosan. The isotherm and kinetics of Hg²⁺/Hg⁺ removal follows Langmuir isotherm and pseudo-second order kinetics, respectively. Besides, a series of fluorescent hydrogels were prepared to compare the elasticity, hydropHilicity, fluorescence intensity and adsorption capacity.