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Related Experiment Video

Updated: Sep 26, 2025

Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids

Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids

Published on: October 7, 2020

Extracellular Microenvironmental Control for Organoid Assembly.

Kathryn M Sullivan¹, Eunkyung Ko¹, Eun Mi Kim²

¹Department of Bioengineering, and University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

Tissue Engineering. Part B, Reviews

|April 22, 2022

Summary

This summary is machine-generated.

Engineered extracellular microenvironments significantly regulate organoid quality. This review details how natural tissue properties inform the design of microenvironments for brain, lung, intestine, liver, retinal, and kidney organoid development.

Keywords:

biomaterials hydrogel microfabrication organoid

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Last Updated: Sep 26, 2025

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Area of Science:

Bioengineering
Tissue Engineering
Developmental Biology

Background:

Organoids mimic organ physiology and are increasingly vital in bioengineering.
The extracellular microenvironment (soluble molecules, ECM, biofluid flow) influences organoid quality.
Optimal microenvironments for specific organoid engineering remain underexplored.

Purpose of the Study:

To review how engineered extracellular microenvironments regulate organoid quality.
To guide researchers in designing organoid microenvironments based on natural tissue properties.
To summarize microenvironmental contributions to specific organoid formation and growth.

Main Methods:

Summarizing natural tissue chemical and mechanical properties.
Reviewing literature on microenvironment-mediated organoid formation and growth.
Discussing organoid formation and evaluation approaches (ECM, culture, analysis).

Main Results:

Natural microenvironment characteristics provide a blueprint for organoid engineering.
Specific microenvironmental factors significantly impact brain, lung, intestine, liver, retinal, and kidney organoid development.
Detailed methods for organoid formation and evaluation are presented.

Conclusions:

Engineered extracellular microenvironments are critical for advancing organoid technology.
Understanding natural tissue cues is essential for successful organoid engineering.
This review provides a comprehensive guide to microenvironment optimization for diverse organoid models.