GALAXY ver3: updated web application for glycosylation profiling based on 3D HPLC map.

Area of Science:

• Glycomics
• Analytical Chemistry
• Biotechnology

Background:

• High-performance liquid chromatography (HPLC) elution data are crucial for quantitative glycosylation profiling and distinguishing isomeric oligosaccharides.
• The GALAXY web application, utilizing a 3D HPLC map of N-linked oligosaccharides, is widely used for N-glycosylation profiling across molecular, cellular, and tissue levels.

Purpose of the Study:

• To introduce GALAXY version 3, an updated web application for glycomic analysis.
• To incorporate new HPLC data, including glucuronylated and sulfated glycans, into the GALAXY platform.
• To enhance user experience and data integration through an improved graphical user interface and links to glycan databases.

Main Methods:

• Utilizing high-performance liquid chromatography (HPLC) elution data for N-linked oligosaccharide analysis.
• Developing an updated web application (GALAXY v3) with a modern graphical user interface.
• Integrating GALAXY v3 with glycan information resources such as GlyTouCan and the GlyCosmos Portal.

Main Results:

• GALAXY version 3 now includes expanded HPLC data for various glycan types, including glucuronylated and sulfated glycans.
• The application features an improved graphical user interface for enhanced usability.
• GALAXY v3 is linked to GlyTouCan and the GlyCosmos Portal, facilitating easier access to glycan information.

Conclusions:

• The updated GALAXY v3 provides a more comprehensive tool for N-glycosylation profiling.
• The integration with glycan databases and multiomics data will advance glycomic analyses in various organisms.
• This advancement supports detailed glycomic studies at molecular, cellular, and tissue levels.