Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

2.8K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.8K
Deleterious Substances in Aggregate01:25

Deleterious Substances in Aggregate

275
Deleterious substances in aggregates can be detrimental to the quality and durability of concrete. These substances include organic impurities like loam, which interfere with cement hydration and are usually present in the sand. These prevent a good bond between aggregate and cement paste. Organic impurities can be detected using the colorimetric test, where the darkness of a solution after agitation indicates the level of organic content.
Another type of impurity is clay and fine material that...
275
Aggregates Classification01:29

Aggregates Classification

398
Aggregate classification is generally based on its size, petrographic characteristics, weight, and source. Size classification ranges from coarse to fine aggregates, defined by the size of the particles. Coarse aggregates are particles that do not pass through ASTM sieve No. 4, and aggregates that pass through the sieve are fine aggregates.
Petrographic classification groups aggregates based on common mineralogical characteristics. Some of the common mineral groups found in aggregates are...
398
Bioremediation00:46

Bioremediation

20.5K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
20.5K
Environmental Applications of Microorganisms01:30

Environmental Applications of Microorganisms

341
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
341
Comparative Excretory Systems02:24

Comparative Excretory Systems

24.6K
Animals have evolved different strategies for excretion, the removal of waste from the body. Most waste must be dissolved in water to be excreted, so an animal’s excretory strategy directly affects its water balance.
24.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Translation in proximity to forming autophagosomes during sustained autophagy.

Nature communications·2026
Same author

A repeat expansion in GOLGA8A is a major risk factor for atypical frontotemporal lobar degeneration with ubiquitin-positive inclusions.

Nature genetics·2026
Same author

Microautophagy: current understanding of its molecular mechanisms and functions.

Autophagy reports·2026
Same author

VMAT2 dysfunction impairs vesicular dopamine uptake, driving its oxidation and α-synuclein pathology in DJ-1-linked Parkinson's neurons.

Science advances·2026
Same author

TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1.

Science advances·2026
Same author

TDP-43 Phosphorylation: Pathological Modification or Protective Factor Antagonizing TDP-43 Aggregation in Neurodegenerative Diseases?

BioEssays : news and reviews in molecular, cellular and developmental biology·2025
Same journal

Genome-wide rotational and translational phasing of nucleosomes with human transcription factors.

Molecular cell·2026
Same journal

Spliceosomal proofreading factors safeguard 3' splice-site fidelity and prevent proteotoxicity and inflammation.

Molecular cell·2026
Same journal

Cytosolic EZH2-IMPDH2 complexes regulate melanoma progression and metastasis via GTP.

Molecular cell·2026
Same journal

A bacterial reverse transcriptase: Protein-templated DNA synthesis fuels antiviral immunity.

Molecular cell·2026
Same journal

Tweezing apart ribosome heterogeneity.

Molecular cell·2026
Same journal

An NADPH safety valve: De novo lipogenesis buffers biguanide-induced reductive stress.

Molecular cell·2026
See all related articles

Related Experiment Video

Updated: Sep 26, 2025

The Effect of Construction and Demolition Waste Plastic Fractions on Wood-Polymer Composite Properties
09:06

The Effect of Construction and Demolition Waste Plastic Fractions on Wood-Polymer Composite Properties

Published on: June 7, 2020

8.2K

Only solid waste, please!

Dorothee Dormann1, Christian Behrends2

  • 1Johannes Gutenberg University (JGU) Mainz, Faculty of Biology, Institute of Molecular Physiology, Mainz, Germany; Institute of Molecular Biology (IMB) Mainz, Mainz, Germany.

Molecular Cell
|April 22, 2022
PubMed
Summary
This summary is machine-generated.

Researchers discovered that CCT2 acts as a receptor for aggrephagy, a process clearing solid protein aggregates. This mechanism is ubiquitin-independent and distinguishes solid aggregates from liquid condensates.

More Related Videos

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste
08:14

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste

Published on: July 18, 2025

382
Author Spotlight: Advancing Anaerobic Microbiota Research Using a Novel Respirometry Protocol
06:11

Author Spotlight: Advancing Anaerobic Microbiota Research Using a Novel Respirometry Protocol

Published on: April 26, 2024

1.4K

Related Experiment Videos

Last Updated: Sep 26, 2025

The Effect of Construction and Demolition Waste Plastic Fractions on Wood-Polymer Composite Properties
09:06

The Effect of Construction and Demolition Waste Plastic Fractions on Wood-Polymer Composite Properties

Published on: June 7, 2020

8.2K
Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste
08:14

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste

Published on: July 18, 2025

382
Author Spotlight: Advancing Anaerobic Microbiota Research Using a Novel Respirometry Protocol
06:11

Author Spotlight: Advancing Anaerobic Microbiota Research Using a Novel Respirometry Protocol

Published on: April 26, 2024

1.4K

Area of Science:

  • Cellular Biology
  • Molecular Mechanisms
  • Autophagy Research

Background:

  • Selective autophagy, or aggrephagy, is crucial for clearing toxic protein aggregates.
  • The precise receptors and mechanisms mediating aggrephagy remain incompletely understood.
  • Distinguishing the clearance of solid aggregates from liquid-like condensates is an active area of investigation.

Purpose of the Study:

  • To identify the specific receptor mediating the selective clearance of solid protein aggregates via aggrephagy.
  • To elucidate the mechanism by which this receptor recognizes and targets protein aggregates.
  • To determine if the identified mechanism differentiates between solid aggregates and liquid-like condensates.

Main Methods:

  • Utilized yeast models and biochemical assays.
  • Investigated the role of chaperonin TRiC subunit CCT2 in protein aggregate clearance.
  • Analyzed the ubiquitination status of aggregates during clearance.

Main Results:

  • Identified chaperonin TRiC subunit CCT2 as a key receptor for aggrephagy.
  • Demonstrated that CCT2 specifically promotes the clearance of solid protein aggregates.
  • Showed that CCT2-mediated clearance occurs independently of ubiquitin signaling.
  • Confirmed that CCT2 does not promote the clearance of liquid-like condensates.

Conclusions:

  • Chaperonin TRiC subunit CCT2 acts as a specific receptor in aggrephagy.
  • CCT2 mediates the ubiquitin-independent clearance of solid protein aggregates.
  • This finding provides new insights into the selective nature of autophagy in protein quality control.