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Development of Blood Vessels

Development of Blood Vessels

The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...

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Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

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Published on: October 21, 2013

Multi-Layered Human Blood Vessels-on-Chip Design Using Double Viscous Finger Patterning.

Elise Delannoy^1,2, Géraldine Tellier^1,3, Juliette Cholet^1,3

¹CNRS/IIS/Centre Oscar Lambret/Lille University SMMiL-E Project, CNRS Délégation Hauts-de-France, 43 Avenue le Corbusier, 59800 Lille, France.

|April 23, 2022

Summary

Researchers developed a new blood vessel-on-chip model for high-throughput drug screening. This model, using viscous finger patterning, creates realistic vascular structures that improve drug testing efficiency and accuracy.

Keywords:

BioMEMS blood vessels inflammation microfluidics organs-on-chips permeability

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Published on: May 17, 2021

Area of Science:

Biomedical Engineering
Vascular Biology
Drug Discovery

Background:

Vascular function research requires efficient methods for biological replicates.
Existing blood vessel-on-chip models face limitations in medium- to high-throughput screening.
Reproducing complex vascular structures is crucial for accurate drug testing.

Purpose of the Study:

To develop an efficient method for creating blood vessel-on-chip models for high-throughput screening.
To engineer multi-layered, structurally accurate blood vessels using polydimethylsiloxane-glass chips.
To investigate the role of perivascular cells in vascular barrier function and drug response.

Main Methods:

Utilized viscous finger patterning to create channels of varying diameters in collagen solution.
Developed a technique for simultaneous cell seeding to form concentric endothelial and perivascular cell layers.
Established a multi-vessel chip format compatible with standard multiwell plates for parallel screening.

Main Results:

Successfully created structurally correct blood vessels-on-chips with distinct endothelial and perivascular layers.
Demonstrated a tight vascular barrier with intact adherens junctions in the engineered vessels.
Showed that perivascular cells significantly enhance vascular barrier tightness and reduce thrombin-induced permeability.

Conclusions:

The developed blood vessel-on-chip model is suitable for medium- to high-throughput drug screening.
Perivascular cells play a critical role in regulating vascular barrier properties.
This technology enables real-time assessment of drug effects on multicellular vascular models.