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Application of Fucoidan in Caco-2 Model Establishment
Qiong Yang1, Maochen Xing1, Ke Wang1
1Marine College, Shandong University, Weihai 264209, China.
Pharmaceuticals (Basel, Switzerland)
|April 23, 2022
Summary
Fucoidan accelerates Caco-2 model development for intestinal absorption studies. This novel method significantly reduces the Caco-2 cell model establishment time to just 5 days, lowering experimental costs.
Area of Science:
- Biotechnology
- Pharmacology
- Cell Biology
Background:
- The Caco-2 cell model is crucial for in vitro intestinal absorption studies.
- Standard Caco-2 model establishment takes 21 days, with puromycin (PM) reducing it to 7 days.
- Shorter modeling times are needed to decrease experimental costs and enhance model application.
Purpose of the Study:
- To investigate fucoidan's efficacy in shortening Caco-2 model establishment time.
- To evaluate the quality of Caco-2 models established using fucoidan.
Main Methods:
- Caco-2 cells were treated with fucoidan and puromycin.
- Transepithelial electrical resistance (TEER) was measured.
- Alkaline phosphatase (AKP) activity, fluorescein sodium transmittance, and cell morphology were assessed.
Main Results:
- Fucoidan combined with puromycin enabled TEER values >500 Ω·cm2 by day 4.
- AKP activity, fluorescein sodium transmittance, and cell morphology met model establishment criteria.
- Fucoidan did not impede the absorption of macromolecules or drugs.
Conclusions:
- Fucoidan effectively shortens Caco-2 model establishment to 5 days.
- This method provides a cost-effective and efficient approach for Caco-2 model development.
- Fucoidan is a viable agent for rapid Caco-2 model generation.
