Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Machine learning-driven QSAR modeling combined with single cell transcriptomics identifies novel drug targets for lung cancer.

Journal of translational medicine·2026
Same author

Biopsychosocial Influences on the Gut Microbiome in Women's Health: Moving Towards Eubiosis.

Behavioral sciences (Basel, Switzerland)·2026
Same author

Delineating the effects of prenatal oxycodone exposure and melatonin treatment on placental and fetal outcomes in pregnant rats.

bioRxiv : the preprint server for biology·2026
Same author

Melatonin Partially Attenuates Oxycodone-Induced Placental Stress Signaling and Fetal Brain Apoptosis in a Sex-Specific Manner.

bioRxiv : the preprint server for biology·2026
Same author

VST-DAVis: an R Shiny application and web-browser for spatial transcriptomics data analysis and visualization.

Bioinformatics advances·2026
Same author

scAED: a framework for mapping the enhancer state at single-cell resolution.

Briefings in bioinformatics·2025

Related Experiment Video

Updated: Sep 26, 2025

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning
10:36

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

Published on: December 15, 2016

10.7K

Decoding the Synaptic Proteome with Long-Term Exposure to Midazolam during Early Development.

Nghi M Nguyen1,2, Neetha N Vellichirammal2, Chittibabu Guda2

  • 1Department of Anesthesiology, University of Nebraska Medical Center, Omaha, NE 68198, USA.

International Journal of Molecular Sciences
|April 23, 2022
PubMed
Summary
This summary is machine-generated.

Long-term exposure to midazolam (MDZ) in neonatal rodents alters synaptic proteins, potentially impacting neurodevelopment. This study identifies alpha adducin as a key protein affected by early-life sedative exposure.

Keywords:
NICUalpha adducinmidazolamneurodevelopment proteomicssynaptosomes

More Related Videos

Author Spotlight: Exploring Glial Influence in Experience-Dependent Synaptic Pruning During Critical Periods
07:13

Author Spotlight: Exploring Glial Influence in Experience-Dependent Synaptic Pruning During Critical Periods

Published on: March 1, 2024

782
Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient
08:30

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Published on: September 17, 2011

31.9K

Related Experiment Videos

Last Updated: Sep 26, 2025

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning
10:36

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

Published on: December 15, 2016

10.7K
Author Spotlight: Exploring Glial Influence in Experience-Dependent Synaptic Pruning During Critical Periods
07:13

Author Spotlight: Exploring Glial Influence in Experience-Dependent Synaptic Pruning During Critical Periods

Published on: March 1, 2024

782
Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient
08:30

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Published on: September 17, 2011

31.9K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Pharmacology

Background:

  • Neonatal intensive care units (NICU) frequently use sedatives like midazolam (MDZ).
  • Concerns exist regarding potential neurodevelopmental risks from early-life sedative exposure.
  • Previous studies link single midazolam exposures to learning deficits, but long-term effects on synaptic function are unclear.

Purpose of the Study:

  • To investigate the impact of prolonged midazolam (MDZ) exposure on synaptic alterations in early development.
  • To identify proteomic signatures associated with long-term MDZ exposure in a preclinical rodent model.

Main Methods:

  • A dose-escalation regimen of midazolam (MDZ) was administered to rodent pups from postnatal day 3 to 21.
  • Quantitative mass-spectrometry-based proteomics was performed on purified synaptosomes from control and MDZ-exposed animals.
  • Bioinformatic analysis (ClueGO) and Western blot were used to identify and validate protein changes.

Main Results:

  • Proteomic analysis revealed enrichment of proteins involved in actin-binding and protein depolymerization.
  • Alpha adducin (ADD1), a cytoskeleton protein, was significantly upregulated in the midazolam-exposed group.
  • Western blot confirmed the increased expression of alpha adducin (ADD1) following long-term MDZ exposure.

Conclusions:

  • Long-term midazolam (MDZ) exposure during early development induces synaptic alterations.
  • Upregulation of alpha adducin (ADD1) suggests a role in MDZ-induced synaptic changes.
  • These synaptic perturbations may contribute to later neurobehavioral deficits.