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Related Experiment Videos

Protease production with sweet potato residue by solid state fermentation.

S S Yang, W F Chiu

    Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi = Chinese Journal of Microbiology and Immunology
    |November 1, 1986
    PubMed
    Summary

    Optimal conditions for protease production using Aspergillus niger NTU-AM-1 were identified through solid-state fermentation of sweet potato residue. The resulting protease exhibited thermal stability and specific activity.

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    Area of Science:

    • Biotechnology
    • Enzyme Technology
    • Microbial Fermentation

    Background:

    • Sweet potato residue is an abundant agricultural byproduct.
    • Enzyme production, particularly proteases, is crucial for various industrial applications.
    • Solid-state fermentation (SSF) offers an eco-friendly approach for microbial enzyme production.

    Purpose of the Study:

    • To optimize solid-state fermentation conditions for protease production by Aspergillus niger NTU-AM-1.
    • To characterize the properties of the produced protease.
    • To evaluate the potential of using sweet potato residue as a substrate.

    Main Methods:

    • Solid-state fermentation of sweet potato residue supplemented with rice bran and minerals.
    • Optimization of fermentation parameters including moisture content, pH, temperature, and incubation time.

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  • Enzyme recovery through shaking and extraction with NaCl solution.
  • Partial purification using DEAE-Sephacel column chromatography.
  • Enzyme activity assays and stability studies at various pH and temperatures.

    • Main Results:

    • Optimal protease production achieved at 50-58% initial moisture content, pH 3.5-4.3, 20-26°C, and 5 days incubation.
    • Protease yield of 814 units per gram dry weight of substrate.
    • Partially purified protease demonstrated thermal stability, retaining 80-100% activity at 55°C for 40 minutes within pH 4.0-5.5.
    • Enzyme activity was stimulated by EDTA and cysteine, and inhibited by HgCl2.

    Conclusions:

    • Sweet potato residue is a suitable substrate for protease production by Aspergillus niger NTU-AM-1 via SSF.
    • The optimized fermentation process yields a thermally stable protease with potential industrial applications.
    • Further research can explore large-scale production and diverse applications of this enzyme.