Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Etching on the edge: enamel loss under repeated and active HCl applications as a resin infiltration pretreatment.

Journal of applied oral science : revista FOB·2025
Same author

On-Chip Polarization Light Microscopy.

Biosensors·2025
Same author

Fault Detection and Diagnosis in Industry 4.0: A Review on Challenges and Opportunities.

Sensors (Basel, Switzerland)·2025
Same author

Identifying the Molecular Fingerprint of Beta-Lactams via Raman/SERS Spectroscopy Using Unconventional Nanoparticles for Antimicrobial Stewardship.

Antibiotics (Basel, Switzerland)·2025
Same author

High-performance plasmonics nanostructures in gas sensing: a comprehensive review.

Medical gas research·2024
Same author

Tailoring Plasmonic Nanoheaters Size for Enhanced Theranostic Agent Performance.

Bioengineering (Basel, Switzerland)·2024

Related Experiment Video

Updated: Sep 26, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.6K

Optimizing and Quantifying Gold Nanospheres Based on LSPR Label-Free Biosensor for Dengue Diagnosis.

Sajid Farooq1,2, Faiz Wali3, Denise Maria Zezell1

  • 1Center for Lasers and Applications, Instituto de Pesquisas Energeticas e Nucleares, IPEN-CNEN, Sao Paulo 05508-000, Brazil.

Polymers
|April 23, 2022
PubMed
Summary

This study introduces a novel, label-free immunosensor using gold nanoparticles for precise dengue NS1 antigen detection. The optimized sensor achieves a low limit of quantification, offering a new approach for biosensing applications.

Keywords:
figure of meritnanosensorplasmonicsensitivity

More Related Videos

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.7K
Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection
06:00

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Published on: January 26, 2024

1.5K

Related Experiment Videos

Last Updated: Sep 26, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.6K
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.7K
Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection
06:00

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Published on: January 26, 2024

1.5K

Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Optical Sensing

Background:

  • Localized surface plasmon resonance (LSPR) is crucial for sensing, with efficiency depending on refractive index sensitivity (ηRIS) and spectral width (FWHM).
  • Complex nanostructures offer good ηRIS but often have wide LSPR spectra, limiting detection sensitivity.
  • Developing precise, label-free biosensors requires optimizing LSPR features for high sensitivity and narrow spectral peaks.

Purpose of the Study:

  • To develop a facile, label-free, solution-based LSPR immunosensor.
  • To optimize gold nanoparticle (Au NP) size for enhanced sensing performance.
  • To evaluate the sensor's capability for detecting dengue NS1 antigens.

Main Methods:

  • Utilized 3D full-wave field analysis to evaluate optical properties and optimize Au NP size.
  • Investigated the effect of Au NP radius (5-50 nm) on spectral resonance and LSPR parameters.
  • Employed Campbell's model for evaluating molecular sensing performance and applied the platform for dengue NS1 antigen detection.

Main Results:

  • Optimized Au NP radius (30 nm) yielded a high figure of merit (FoM) of 2.3 and a sharp FWHM of 44 nm.
  • Demonstrated a red-shift in spectral resonance from 520 nm to 552 nm with increasing Au NP size.
  • Achieved a limit of quantification of 0.07 μg/mL (1.50 nM) for dengue NS1 antigens.

Conclusions:

  • The optimized Au NP-based LSPR immunosensor offers high precision and sensitivity for molecular detection.
  • This approach provides a new paradigm for engineering efficient and label-free immunosensor platforms.
  • The developed sensor demonstrates significant potential for clinical diagnostics, particularly for infectious diseases like dengue.