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Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
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An Inducible Microbacterium Prophage vB_MoxS-R1 Represents a Novel Lineage of Siphovirus

Hongrui Zheng1, Binbin Liu1, Yongle Xu1,2

  • 1Institute of Marine Science and Technology, Shandong University, Qingdao 266000, China.

Viruses
|April 23, 2022
PubMed

Insights

This study details the siphovirus vB_MoxS-R1, induced from Microbacterium. Its genome contains genes for stress response, phosphorus acquisition, and DNA recombination, potentially influencing host adaptation and evolution.

Area of Science:

  • Virology
  • Microbial Genetics
  • Marine Microbiology

Background:

  • Viruses employ lytic and lysogenic infections to interact with microbial hosts.
  • Prophage genetic information offers insights into phage characteristics and host influences.
  • Estuarine Synechococcus cultures serve as a source for isolating microbial hosts and their associated phages.

Purpose of the Study:

  • To characterize the siphovirus vB_MoxS-R1, induced from a Microbacterium strain.
  • To perform a novel phage genus-based genomic analysis of vB_MoxS-R1.
  • To investigate the potential roles of predicted genes in host-phage interactions and host evolution.

Main Methods:

  • Induction of the siphovirus vB_MoxS-R1 from a Microbacterium host.
  • Genomic analysis of the vB_MoxS-R1 phage.
  • Bioinformatic prediction of genes and their functions within the phage genome.

Main Results:

  • vB_MoxS-R1 exhibits high replication capability (burst size ~2000 virions/cell).
  • The genome contains six transcriptional regulator (TR) genes, four linked to bacterial stress, virulence, and amino acid transport.
  • A glycerophosphodiester phosphodiesterase gene for phosphorus acquisition and four endonuclease genes were identified.

Conclusions:

  • Prophage vB_MoxS-R1's genetic makeup suggests potential roles in regulating host metabolism and adaptation during lysogeny.
  • The identified genes indicate vB_MoxS-R1 may influence host survival, adaptation, and evolution through DNA recombination and gene conversion.
Keywords:
Microbacterium prophagecomparative genomic analysisendonucleasenew phage genustranscriptional regulator

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