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Related Experiment Video

Updated: Sep 26, 2025

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

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Published on: July 17, 2012

High-sensitivity dynamic diffuse fluorescence tomography system for fluorescence pharmacokinetics.

Limin Zhang^1,2, Nan Cheng^1,3, Han Liu¹

¹Tianjin University, College of Precision Instrument and Optoelectronics Engineering, Tianjin, China.

Journal of Biomedical Optics

|April 23, 2022

Summary

This summary is machine-generated.

A new dynamic diffuse fluorescence tomography (DFT) system effectively tracks indocyanine green (ICG) metabolism in vivo. This advancement aids in tumor detection, drug assessment, and liver function evaluation using dynamic imaging.

Keywords:

diffuse fluorescence tomography dynamic measurement indocyanine green lock-in photon counting

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Area of Science:

Biomedical optics
Medical imaging
Fluorescence tomography

Background:

Dynamic diffuse fluorescence tomography (DFT) enables tracking of physiological and disease progression.
Indocyanine green (ICG) dynamic imaging is underutilized due to low quantum yield and rapid metabolism.

Purpose of the Study:

To develop and validate a dynamic DFT system for indocyanine green (ICG) pharmacokinetic analysis.
To assess the system's capability for dynamic ICG imaging in biological applications.

Main Methods:

A fiber-based dynamic DFT system utilizing square-wave modulation lock-in photon counting and series-parallel measurement.
Assessment of measurement stability and anti-crosstalk performance.
Validation through static phantoms, dynamic phantoms, and in vivo mouse experiments.

Main Results:

The system demonstrates high sensitivity, a 100 dB dynamic range, and low crosstalk (<1.35%).
Accurate reconstruction of target positions, sizes, and shapes in static phantoms.
Successful capture of fast fluorescence signals in dynamic phantoms and tracking of ICG metabolism in vivo.

Conclusions:

The proposed dynamic DFT system is effective for assessing ICG pharmacokinetics.
This technology offers a valuable tool for tumor detection, drug assessment, and liver function evaluation.