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Updated: Jul 9, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
Published on: June 29, 2017
Genomic analysis, simultaneous production, and process optimization of extracellular polymeric substances and
Bhawna Tyagi1, Bulbul Gupta2, Deepak Khatak1
1School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Abstract:
In the current study, the isolated Methylobacterium sp. ISTM1 simultaneously produced both extracellular polymeric substances (EPS) and polyhydroxyalkanoates (PHA) in a single-step process. The yield of biopolymers (EPS and PHA) was enhanced by optimizing the process parameters of EPS and PHA production. Methylobacterium sp. ISTM1 was able to produce 7.18 ± 0.04 g L-1 EPS and 1.41 ± 0.04 g L-1 PHA simultaneously at optimized culture conditions i.e., 9% molasses and pH 7. The genomic analysis of the strain has identified the involved genes and pathways in the production of EPS and PHA. Both the biopolymers were found non-toxic according to the cytotoxicity analysis. The results of the current study present the potential of the bacterium Methylobacterium sp. ISTM1 produces non-toxic biopolymers by utilizing agro-industrial waste (molasses) that can be harnessed sustainably for various applications.
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