Genomic analysis, simultaneous production, and process optimization of extracellular polymeric substances and polyhydroxyalkanoates by Methylobacterium sp. ISTM1 by utilizing molasses
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Published on: June 29, 2017
Bhawna Tyagi1, Bulbul Gupta2, Deepak Khatak1
1School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Abstract:
In the current study, the isolated Methylobacterium sp. ISTM1 simultaneously produced both extracellular polymeric substances (EPS) and polyhydroxyalkanoates (PHA) in a single-step process. The yield of biopolymers (EPS and PHA) was enhanced by optimizing the process parameters of EPS and PHA production. Methylobacterium sp. ISTM1 was able to produce 7.18 ± 0.04 g L-1 EPS and 1.41 ± 0.04 g L-1 PHA simultaneously at optimized culture conditions i.e., 9% molasses and pH 7. The genomic analysis of the strain has identified the involved genes and pathways in the production of EPS and PHA. Both the biopolymers were found non-toxic according to the cytotoxicity analysis. The results of the current study present the potential of the bacterium Methylobacterium sp. ISTM1 produces non-toxic biopolymers by utilizing agro-industrial waste (molasses) that can be harnessed sustainably for various applications.
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