Length- and polymer-dependent ecotoxicities of microfibers to the earthworm Eisenia andrei

Microfibers are widespread environmental pollutants introduced to the soil environment because of the increasing use of plastic polymers. However, research on the soil ecotoxicity of microfibers is limited, especially when compared to research on their aquatic toxicity. This study investigated the effects of sub-chronic microfiber exposure on the earthworm Eisenia andrei. We compared the effects of three types of microfibers: short lyocell microfibers (231 ± 126 μm long), short polypropylene microfibers (191 ± 107 μm long), and long polypropylene microfibers (891 ± 141 μm long). After exposure for 21 days, earthworm survival, coelomocyte viability, cast microbial viability, and gut microbial viability were assessed, and a histopathological examination of the digestive tract and reproductive tissues was conducted. In addition, long polypropylene microfibers egested by the earthworms were collected to explore the possibility of earthworm-driven biofragmentation. Results indicated that high exposure concentration (1000 mg/kg dry soil) negatively affected earthworm coelomocytes and intestinal tissue, gut, and cast microbiomes. Although all three microfiber types reduced earthworm survival, the short polypropylene microfibers were more toxic to the earthworms than the long polypropylene microfibers or short lyocell microfibers, which indicated that size-dependent soil ecotoxicity was induced. PP microfibers were found to more negatively affect cast microbial activity and intestinal tissue than lyocell microfibers, indicating polymer-dependent soil ecotoxicity potential against earthworm species. This study provides evidence that synthesized microfibers cause cytotoxicity and decrease gut microbiome viability in earthworms, and that they can be biofragmented by earthworms.