Interrogating Global Chromatin Interaction Network by High-Throughput Chromosome Conformation Capture (Hi-C) in
Wenjing Wang1,2, Longjian Niu1, Chunhui Hou3
1Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China.
Methods in Molecular Biology (Clifton, N.J.)
|April 24, 2022
Abstract:
High-throughput chromosome conformation capture (Hi-C) enables the global quantification of chromatin interaction frequency in eukaryotic nuclei. This method is based on in situ Hi-C, in which chromatin is cross-linked with formaldehyde, then digested with restriction enzyme. Biotin-labeled nucleotide is incorporated before the spatially adjacent DNA ends are ligated, making it possible to enrich specifically the chimeric ligation products for deep sequencing. In this chapter, we describe a modified in situ Hi-C protocol for the global chromatin interaction analysis in plants.
