Magnetic layered double hydroxide nanosheet as a biomolecular vessel for enzyme immobilization

Magnetic nanoparticle coated with manganese‑aluminum layered double hydroxide (Fe3O4/Mg-Al-CO3-LDH) was prepared and used as porous support for ficin (EC 3.4.22.3) as a model enzyme. Structural characteristics were studied by XRD, FTIR, SEM and light scattering. The quantity of immobilized ficin on the mentioned LDH and non-magnetic LDH was measured and enzyme activity, stability and reusability were compared. Results revealed that the core and shell structure of Fe₃O₄/Mg-Al-CO₃-LDH makes it better dispersion compared to the pristine Mg-Al-CO₃-LDH. Ficin showed strong affinity to absorption of the surface of mentioned LDHs nanosheet especially magnetic LDH, confirmed that the existence of Fe₃O₄ in the core structure of magnetic Fe₃O₄/Mg-Al-CO₃-LDH caused better dispersion of LDH nanocrystal shell compared to pristine LDH moreover, enzyme which immobilized on the magnetic LDH supports, can be recovered by magnetic interaction. The storage stability of free ficin, immobilized ficin on the Mg-Al-CO₃-LDH and Fe₃O₄/Mg-Al-CO₃-LDH during a period of 120 days lost about 75%, 30%, and 20% of their initial activities, respectively.