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Binding properties of [Ru(phen)2(11-R-dppz)]2+ (R = F or CN) with poly(A)•poly(U) duplex RNA
Wei Li1, Xiaohua Liu2, Li-Feng Tan3
1College of Chemistry, Xiangtan University, Xiangtan 411105, People's Republic of China.
Abstract:
Two Ru(II) complexes, [Ru(phen)2(11-F-dppz)]2+ (Ru1, phen = 1,10-phenanthroline, 11-F-dppz = 11-fluorodipyrido[3,2-a:2',3'-c]phenazine) and [Ru(phen)2(11-CN-dppz)]2+ (Ru2, 11-CN-dppz = 11-cyanodipyrido[3,2-a:2',3'-c]phenazine), have been synthesized and characterized in this work. The binding properties of Ru1 and Ru2 with poly(A)•poly(U) RNA duplex have been investigated by spectroscopic methods and viscosity measurements. UV-vis absorption spectra and viscosity experiments demonstrate that the binding modes of Ru1 and Ru2 with poly(A)•poly(U) RNA duplex are intercalation, while the binding affinity for Ru2 is greater than that for Ru1. In addition, thermal denaturation studies reveal that both complexes significantly improve the stability of poly(A)•poly(U) duplex RNA. However, fluorescence titrations indicate that Ru1, unlike Ru2, can act as a molecular "light switch" for the poly(A)•poly(U) duplex RNA. The obtained results of this work indicate that the electron-withdrawing effect of substituents on the main ligands can significantly affect the binding of Ru(II) polypyridyl complexes with poly(A)•poly(U).
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