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Generation of an induced pluripotent stem cell line (ZZUi034-A) from a 65 year old Chinese female donor with sendai

Yamin Zhang1, Jing Zhu2, Yaozhang Dai1

  • 1Department of Otorhinolaryngology Head and Neck Surgery, the 1(st) Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

Stem Cell Research
|April 24, 2022
PubMed

Peripheral blood was extracted from a 65 year old Chinese female health donor. Induced pluripotent stem cells (iPSC) were reprogrammed with the 4 reprogramming factors: Klf-4, c-Myc, Oct-4, Sox-2, using sendai virus reprogramming protocol. The iPSC line showed pluripotency which was confirmed by immunofluorescent staining; the iPSC line was also able to form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This healthy iPSC line can be served as healthy control for disease mechanism and disease modeling study.

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