Dynamics of the Bacterial Community's Soil During the In-Situ Degradation Process of Waste Chicken Feathers

Bacteria play an important role in the biodegradation of feather waste. The exploration of the related microbial community structure and diversity is essential to improve the performance of feather waste treatment processes. In the present work, an in-situ soil sampled from a poultry farm was directly used to simulate and accelerate the natural degradation processes of feather waste under laboratory conditions, in which the dynamics of the microbial communities was further analyzed by Illumina HiSeq high-throughput 16S rRNA gene sequencing. Biochemical factors, including pH, feather degradation rate and soluble protein content were also determined in this study. The biochemical results showed that the in-situ soil exhibited an effective degradability on chicken feathers, and the degradation rate of feathers reached 57.95 ± 3.09% at 120 h of cultivation. Meanwhile, soluble protein content and pH reached 33.62 ± 1.45 mg/mL 8.99 ± 0.08, respectively. The results of bacterial diversity analysis showed that bacterial community structure and composition significantly varied in each phase of degradation. Additionally, the bacteria system with feather degradability might consist of Bacillus, Chryseobacterium, Lysobacter, Brevibacillus, and Stenotrophomonas genera. This system may include the following key pathways: carbohydrate metabolism, amino acid metabolism, nucleotide metabolism, membrane transport, replication and repair, translation, signal transduction and energy metabolism. Moreover, the bacterial communities may occur community succession during the degradation processes of chicken feathers. In summary, the present work provided valuable insights into the understanding of microbial community and metabolic functions for feather degradation, although the in-situ biodegradation process was conducted under laboratory conditions.

The online version contains supplementary material available at 10.1007/s12088-021-00996-6.