Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Slow myosin in developing rat skeletal muscle.

M Narusawa, R B Fitzsimons, S Izumo

    The Journal of Cell Biology
    |March 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The influence of circulating cholesterol and its components in middle-aged adults on cognitive function at mid- and later-life; a systematic review.

    Frontiers in aging·2025
    Same author

    Hypothalamic vasopressin neural densities are higher in male Mongolian gerbils after separation from a pair bond partner and may facilitate behavior to form a new bond.

    Behavioural brain research·2024
    Same author

    The ethical matrix as a method for involving people living with disease and the wider public (PPI) in near-term artificial intelligence research.

    Radiography (London, England : 1995)·2023
    Same author

    Therapeutic vaccination based on side population cells transduced by the granulocyte-macrophage colony-stimulating factor gene elicits potent antitumor immunity.

    Cancer gene therapy·2017
    Same author

    Platelet responses to agonists in a cohort of highly characterised platelet donors are consistent over time.

    Vox sanguinis·2016
    Same author

    Temporary nasal obstruction in an infant of a diabetic mother.

    Irish journal of medical science·2016

    Slow myosin heavy-chain (MHC) synthesis begins early in skeletal muscle development, concurrently with embryonic myosin. Fiber type differences emerge due to developmental inhibition of slow MHC accumulation, influenced by muscle assembly and innervation.

    Area of Science:

    • Developmental Biology
    • Muscle Physiology
    • Skeletal Myogenesis

    Background:

    • Skeletal muscle development involves the precise expression of myosin heavy-chain (MHC) isoforms.
    • Understanding the temporal and spatial regulation of MHC expression is crucial for comprehending muscle differentiation.
    • The roles of innervation and developmental timing in establishing muscle fiber type heterogeneity remain areas of active investigation.

    Purpose of the Study:

    • To investigate the early expression patterns of slow myosin heavy-chain (MHC) during fetal skeletal myogenesis.
    • To elucidate the mechanisms underlying the development of fiber type heterogeneity in hind limb muscles.
    • To examine the influence of neonatal denervation and thyroid function on slow MHC accumulation.

    Main Methods:

    Related Experiment Videos

  • S1 nuclease mapping using a specific cDNA probe to detect slow MHC gene expression.
  • Immunofluorescence and Western blotting with a monoclonal antibody specific to adult slow MHC.
  • Radioimmunoassay (RIA) to quantify slow MHC proportions and studies involving neonatal denervation and induced hypothyroidism.

    • Main Results:

    • Slow MHC is expressed in fetal hind limb muscle by 18 days gestation, concurrently with embryonic myosin.
    • All primary myotubes express both slow and embryonic myosin at 16 days gestation; fiber heterogeneity arises from developmental inhibition of slow MHC accumulation.
    • Neonatal denervation leads to decreased slow MHC in soleus but increased slow MHC in extensor digitorum longus (EDL) and anterior tibial (AT) muscles, suggesting an inhibitory role of the nerve in fast-twitch muscles.

    Conclusions:

    • Slow myosin synthesis is an early event in skeletal myogenesis, preceding the establishment of distinct fiber types.
    • Fiber type specialization is regulated by a developmentally programmed inhibition of slow MHC accumulation, influenced by muscle assembly and innervation.
    • The nervous system plays a critical role in modulating slow MHC expression, with differential effects on slow-twitch (soleus) and fast-twitch (EDL, AT) muscles.