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Updated: Sep 26, 2025
Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
Published on: November 20, 2016
A protocol for CRISPR-mediated activation and repression of human endogenous retroviruses in human pluripotent stem
Vidya Padmanabhan Nair1, Jens Mayer2, Michelle Vincendeau1
1Institute of Virology, Helmholtz Zentrum München, 85764 Neuherberg, Germany.
Abstract:
Human endogenous retroviruses (HERVs) comprise many regulatory elements and can regulate host gene activity at different expression levels via multiple mechanisms. Here, we introduce a step-by-step protocol to activate or repress transcription of HERV-K(HML-2) elements using the CRISPRa and CRISPRi technologies in human embryonic stem cells. This protocol can help deciphering the functional role of HERV-K(HML-2) elements in critical biological processes. The protocol may easily be adapted to other cell lines and HERV groups with relatively low sequence heterogeneity. For complete details on the use and execution of this protocol, please refer to Padmanabhan Nair et al. (2021).
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