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Updated: Sep 26, 2025

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
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The Evolution of Intein-Based Affinity Methods as Reflected in 30 years of Patent History

Sai Vivek Prabhala1, Izabela Gierach2, David W Wood1,2

  • 1William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, United States.

Frontiers in Molecular Biosciences
|April 25, 2022
PubMed

Keywords:
inteinspatentsprotein engineeringprotein purificationsplit inteins

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