Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

3.7K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.7K
Leaky Scanning02:28

Leaky Scanning

5.3K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.3K
Conjugated Proteins02:50

Conjugated Proteins

18.7K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TLR7 signaling aggravates lung inflammation associated with increased anti-Scl-70 autoantibody production in murine bleomycin-induced systemic sclerosis.

Frontiers in immunology·2026
Same author

Epitranscriptomic circuits wiring the tumor microenvironment: A pharmacodynamic framework for clinical translation.

Pharmacological research·2026
Same author

Persistent activation of monocytes/macrophages and cell senescence in SIV-infected macaques on ART.

Frontiers in immunology·2026
Same author

Administration of Nicotinamide Mononucleotide Mitigates the HIV Nef-Induced Metabolic and Pathological Changes in the Heart.

Cells·2026
Same author

Targeting TRAF3IP2 disrupts cellular energetics through inhibition of NAMPT in triple negative breast cancer.

Scientific reports·2025
Same author

Persistent Activation of Monocytes/Macrophages and Cell Senescence in SIV-Infected Macaques on ART.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Sep 25, 2025

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.2K

Epitranscriptomics of SARS-CoV-2 Infection.

Amin Izadpanah1, Jay Rappaport1,2, Prasun K Datta1,2

  • 1Division of Comparative Pathology, Tulane National Primate Center, Covington, LA, United States.

Frontiers in Cell and Developmental Biology
|April 25, 2022
PubMed
Summary

This review explores RNA modifications like N6-methyladenosine (m6A) during SARS-CoV-2 infection. Understanding these epitranscriptomic changes offers new therapeutic targets for controlling the virus.

Keywords:
COVID-19RNA modificationsSARS-CoV-2epitranscriptomelung

More Related Videos

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.4K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.4K

Related Experiment Videos

Last Updated: Sep 25, 2025

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.2K
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.4K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.4K

Area of Science:

  • Molecular Biology
  • Virology
  • Epitranscriptomics

Background:

  • SARS-CoV-2 infection involves complex epitranscriptomic modifications.
  • Key RNA modifications include N6-methyladenosine (m6A), pseudouridine (Ψ), and 2'-O-methylation (Nm).

Purpose of the Study:

  • To review recent advances in the epitranscriptomic code of SARS-CoV-2.
  • To discuss the roles of enzymes and proteins involved in RNA modifications during infection.

Main Methods:

  • Literature review of studies on SARS-CoV-2 RNA modifications.
  • Analysis of the roles of m6A readers, writers, and erasers.
  • Examination of pseudouridine synthases and the nsp16/nsp10 complex.
  • Discussion of enzyme expression in infected host cells.

Main Results:

  • m6A, Ψ, and Nm modifications are crucial in SARS-CoV-2 infection.
  • Specific enzymes (pseudouridine synthases, nsp16/nsp10) play key roles.
  • Altered expression of RNA modification enzymes impacts host mRNA.

Conclusions:

  • Understanding RNA modification mechanisms in SARS-CoV-2 is critical.
  • Targeting these epitranscriptomic pathways presents novel therapeutic opportunities.
  • Further research can lead to effective strategies against SARS-CoV-2 infection.