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Updated: Sep 25, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Published on: May 25, 2018

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Chimeric oligonucleotides combining guide RNA and single-stranded DNA repair template effectively induce precision

Avantika Ghosh1, Ksenia Myacheva1,2, Marisa Riester1

  • 1Division of Cancer Research, Department of Thoracic Surgery, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, German Cancer Consortium (DKTK) - Partner Site Freiburg, Freiburg, Germany.

RNA Biology
|April 25, 2022
PubMed
Summary

Chemically synthesized chimeric oligonucleotides enable precise genome editing via CRISPR-Cas9 technology. These RNA-DNA molecules, when fused correctly, facilitate targeted gene modifications for molecular biology and medicine.

Keywords:
CRISPRPrecision Genome EditingRNA

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