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Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
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Orthogonal spin labeling and pulsed dipolar spectroscopy for protein studies

Laura Galazzo1, Markus Teucher2, Enrica Bordignon1

  • 1Department of Physical Chemistry, University of Geneva, Genève, Switzerland.

Methods in Enzymology
|April 25, 2022
PubMed
Summary

This study reviews orthogonal spin labels for Electron Paramagnetic Resonance (EPR) and pulse dipolar spectroscopy (PDS). Combining different labels enhances structural biology insights by enabling simultaneous measurement of multiple distances in biomolecules.

Keywords:
CopperDEERDQCEPRGadoliniumNitroxideOrthogonal labelingPELDORProteinsRIDMESpin labelTrityl

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