Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Complexation Equilibria: The Chelate Effect

Complexation Equilibria: The Chelate Effect

In complexation reactions, metal atoms or cations interact with ligands to form donor-acceptor adducts called metal complexes. Ligands that bind through one donor site are monodentate, ligands with two donor sites are bidentate, and those with more than two donor sites are polydentate ligands. For example, ethylene diamine is a bidentate ligand that binds through two nitrogen donor atoms, forming a five-membered ring. EDTA is a polydentate ligand that binds through four oxygen and two nitrogen...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Molecular Interpretation of Temperature-Induced DNA Melting Monitored through Base Dipole Moment Changes as a Surrogate of the Hypochromic Shift.

The journal of physical chemistry letters·2026

Same author

Agonist-specific conformational dynamics at the β<sub>2</sub>-adrenoceptor dictate allosteric modulation of Gαs signalling and bronchodilation.

British journal of pharmacology·2026

Same author

Comparative evaluation of gene selection approaches in transcriptomics: bias correction and visualization with TransPro.

GigaScience·2026

Same author

Targeting conserved domains of hypoxia-inducible factors for cancer therapy.

The Journal of experimental medicine·2026

Same author

The scientific legacy of Martin Karplus from the perspective of his collaborators.

Biophysical journal·2026

Same author

Computational Ligand-Binding Site Prediction.

Advances in experimental medicine and biology·2026

Search research articles

Related Experiment Video

Updated: Sep 25, 2025

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Published on: March 10, 2021

Cholecalciferol complexation with hydroxypropyl-β-cyclodextrin (HPBCD) and its molecular dynamics simulation.

Fang Wang¹, Wenbo Yu^1,2, Carmen Popescu³

¹Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD, USA.

Pharmaceutical Development and Technology

|April 25, 2022

Summary

This study explores how hydroxypropyl-β-cyclodextrin (HPBCD) can solubilize vitamin D3. Complexation was confirmed using various techniques, leading to a more stable solid dispersion of vitamin D3.

Keywords:

Cholecalciferol (vitamin D3)complex hydroxypropyl-β-cyclodextrin (HPBCD)molecular dynamic (MD) simulations molecular modeling

More Related Videos

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study

Published on: August 15, 2016

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

Published on: August 25, 2023

Related Experiment Videos

Last Updated: Sep 25, 2025

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Published on: March 10, 2021

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study

Published on: August 15, 2016

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

Published on: August 25, 2023

Area of Science:

Pharmaceutical Sciences
Materials Science
Physical Chemistry

Background:

Vitamin D3 (cholecalciferol) is essential but poorly soluble in water.
Cyclodextrins, like HPBCD, are used to improve the solubility of poorly soluble drugs.
Understanding the complexation mechanism is crucial for developing stable formulations.

Purpose of the Study:

To investigate the complexation of vitamin D3 with HPBCD for enhanced solubility.
To characterize the formation and stability of the vitamin D3-HPBCD complex.
To elucidate the molecular interactions involved in the complexation process.

Main Methods:

Phase solubility studies to determine solubility profiles.
Differential Scanning Calorimetry (DSC) to confirm complex formation.
Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) for structural validation.
Scanning Electron Microscopy (SEM) to analyze solid dispersion morphology.
Molecular Dynamics (MD) simulations for computational analysis.

Main Results:

An A_P solubility profile indicated complex formation with increasing HPBCD concentration.
DSC, FTIR, and XRD confirmed the amorphous state of vitamin D3 within the HPBCD complex.
Spray drying yielded a more stable solid dispersion compared to the liquid phase.
MD simulations provided insights into the molecular interactions and thermodynamics of the complex.

Conclusions:

HPBCD effectively complexes with vitamin D3, improving its solubility.
The complex is more stable in a solid dispersion state achieved via spray drying.
Computational modeling complements experimental data, offering a molecular-level understanding of the complex.