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Updated: Sep 25, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
Glutathione S-transferase gene polymorphism and asthma: a case-control study in a pediatric population
Ankit Kumar1, Tanzeel Ahmed1, Mohammad K Parvez2
1School of Biotechnology, IFTM University, Moradabad, Uttar Pradesh, 244102, India.
Abstract:
Aim: To carry out a case-control study of the association of GST gene polymorphisms in pediatric asthma-related oxidative stress. Materials & methods: Asthma patients (n = 250) and age-matched healthy subjects (n = 250) DNA were genotyped for GSTM1/GSTT1 (+/+, +/-, -/+ and -/-) frequencies using multiplex-PCR and plasma oxidative stress markers (examined spectrophotometrically). Results: Asthma patients had significantly more common null-genotype GSTM1 (10.4%; p = 0.002) and elevated levels of malondialdehyde, protein carbonyl and 8-hydroxy-2-deoxyguanosine as compared with controls. In addition, the level of plasma glutathione, GST activity and ferric-reducing ability were significantly decreased as compared with controls. Conclusion: Our data revealed significant associations between GSTM1 genotype and oxidative stress markers in asthmatic children, which may very likely contribute to increased incidence of bronchial asthma.
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