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[Prevention and control of antimicrobial resistance using CRISPR-Cas system: a review]

Chenyu Wang1, Zhizhi Liu1, Biao Tang2

  • 1College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|April 26, 2022
PubMed

Insights

CRISPR-Cas systems offer a novel approach to combat bacterial multi-drug resistance (MDR) by targeting antibiotic resistance genes (ARGs). This technology shows promise for controlling MDR across various sectors.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Bacterial multi-drug resistance (MDR) poses a significant global threat across medicine, agriculture, and environmental sectors.
  • The spread of antibiotic resistance genes (ARGs) is a primary driver of increasing bacterial MDR.
  • Current strategies for combating bacterial MDR are insufficient to address the escalating challenge.

Purpose of the Study:

  • To review the mechanism of CRISPR-Cas systems.
  • To discuss recent advancements in utilizing CRISPR-Cas systems for reducing ARGs.
  • To explore the potential of CRISPR-Cas systems in preventing and controlling bacterial MDR.

Main Methods:

  • Review of the CRISPR-Cas system's working mechanism.
  • Analysis of recent studies on CRISPR-Cas mediated ARG reduction.
  • Discussion of delivery methods for CRISPR-Cas systems, including plasmids, bacteriophages, and nanoparticles.

Main Results:

  • CRISPR-Cas systems can be engineered to target and degrade specific ARGs.
  • Various delivery systems are being explored to enhance the efficiency of CRISPR-Cas based ARG reduction.
  • CRISPR-Cas technology demonstrates significant potential for controlling MDR.

Conclusions:

  • CRISPR-Cas systems represent a promising innovative strategy for combating bacterial MDR.
  • Further research and development in CRISPR-Cas delivery methods are crucial for practical applications.
  • This technology offers a new perspective for preventing and controlling the spread of ARGs and MDR.
Keywords:
CRISPR-Cas systemantibiotic resistance geneshorizontal gene transfermulti-drug resistance

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